THE™ V5 Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.

產品型號：A01724

更新時間：2025-11-06

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產品介紹

產品概述

Specificity	THE™ V5 Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.
Host Species	Mouse
Immunogen	V5 tag peptide conjugated to KLH
Conjugate	Unconjugated

應用指導

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.05-0.1 µg/ml
Western Blot	0.2-1 µg/ml
Immunoprecipitation (IP)	1-5 µg/ml
Flow Cytometry	1-3 µg for 1 x 106 cells
Immunocytochemistry/Immunofluorescence (ICC/IF)	1-3 µg/ml

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產品屬性

Form	Lyophilized
Storage Buffer	lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions	The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles.
Purification	Protein A affinity column
Isotype	Mouse IgG2a,κ
Clonality	Monoclonal
Clone ID	4C12E11