DYKDDDDK Peptide：A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site

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更新時(shí)間：2026-02-27

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分子生物學(xué)

核酸提取純化 CRISPR/Cas9

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Synonyms	DYKDDDDK tag;Flag-tag
Description	A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free DYKDDDDK peptide.
Sequence
Sequence Shortening	DYKDDDDK
Molecular Formula	C41H60N10O20
Molecular Weight	1012.97

產(chǎn)品概述SynonymsDYKDDDDK tag;Flag-tagDescriptionA fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free DYKDDDDK peptide.SequenceSequence ShorteningDYKDDDDKMolecular FormulaC41H60N10O20Molecular Weight1012.97

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