Broad Multi Color Pre-Stained Protein Standard is designed for observing protein separation during SDS-PAGE,

產(chǎn)品型號(hào)：M00624

更新時(shí)間：2026-02-27

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Western Blot產(chǎn)品蛋白純化 ELISA試劑盒抗體內(nèi)毒素檢測(cè)和去除

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產(chǎn)品介紹

Description

Broad Multi Color Pre-Stained Protein Standard is a special-designed protein standard. The standard is a mixture of ten highly purified pre-stained proteins ranging from 10 kDa to 270 kDa, covalently coupled with three different chromophores. The 30 kDa and 270 kDa bands are orange, 50 kDa band is green, and other bands are blue.
Broad Multi Color Pre-Stained Protein Standard is designed for observing protein separation during SDS-PAGE, verifying western transfer efficiency on membranes, and approximating the size of proteins. Due to the influence of covalently coupled chromophores on the electrophoretic mobility of proteins, this product is only suitable for roughly estimating the molecular weight of target protein samples.

產(chǎn)品屬性

Storage Buffer	62.5 mM Tris-H3PO4 (pH 7.5), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol.
Storage	Stable at -20°C for 24 months or 4°C for 3 months. Aliquots are recommended before use to avoid repeated freeze-thaw cycles.
Procedures	1. Thaw this product at room temperature. Do NOT boil protein marker. 2. Mix gently and thoroughly to ensure that the solution is homogeneous. 3. Bands intensity may vary according to gel type, thickness, and staining condition. Please adjust loading volume from 2.5 µl to 10 µl. --> 1. Thaw this product at room temperature. Do NOT boil protein marker. 2. Mix gently and thoroughly to ensure that the solution is homogeneous. 3. Bands intensity may vary according to gel type, thickness, and staining condition. Please adjust loading volume from 2.5 µl to 10 µl.
Recommendations	1. Completely thaw and mix this product at room temperature before using. 2. This product is only suitable for roughly estimating the molecular weight of protein samples. --> 1. Completely thaw and mix this product at room temperature before using. 2. This product is only suitable for roughly estimating the molecular weight of protein samples.

應(yīng)用指導(dǎo)

1.      Monitoring protein migration during SDS-PAGE.
2.      Estimating protein sizes on SDS-PAGE gels.
3.      Verifying transfer efficiency on membranes for Western Blot.
4.      Locating a protein of interest for excision from an unstained preparative gel.

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